Novosibirsk State Pedagogical University Bulletin, 2011, No.1, pp.75-84
UDC: 
577.1

Comparison of methods of allocation metagenomic dna from samples of soil

Galiev V. V. 1 (Novosibirsk, Russian Federation), Tsyrulnikov A. O. 1 (Novosibirsk, Russian Federation)
1 Novosibirsk State Pedagogical University, Novosibirsk, Russian Federation
Abstract: 

In this paper the authors make review and practical comparison of various methods of extraction metagenomic DNA from soil microorganisms. The choice of a method of extraction of DNA is the important part in carrying out of scientific work with metagenomic samples as all further research activity depends on quality of allocated DNA. The authors compared methods of extraction applied to the gray forest soils of the vicinity of Novosibirsk.

Keywords: 

DNA; microorganisms; metagenomic samples; soil

References: 

1. Jia Xia, Han Shi-jie, Zhao Yong-hua, Zhou Yu-mei. Comparisons of extraction and purification methods of soil microorganism DNA from rhizosphere soil // Journal of Forestry Research 2006 – № 17(1). – P. 31–34.
2. Roh, C. H., Villatte, F., Kim, B. G., and Schmid, R. D. // Electrophoresis. – 2005. – V. 26. – P. 3055–3061.
3. Tatiana Volossiouk, E. Jane Robb, and Ross N. Nazar. Direct DNA Extraction for PCR-Mediated Assays of Soil Organisms // Applied and environmental microbiology. – 1995. – P. 3972 –3976.

Date of the publication 29.04.2011