Novosibirsk State Pedagogical University Bulletin, 2013, No.5, pp.23-31
UDC: 
616-008.6:616-002-008.6

Plasma free amino acid profile in patients with oral cancer

Burlaka I. B. 1 (Kiev, Ukraine), Gryn’ N. V. 1 (Kiev, Ukraine), Verevka S. V. 1 (Kiev, Ukraine)
1 Academy of Medical Sciences of Ukraine prof. O.S. Kolomiychenko Institute of Otolaryngology
Abstract: 

Metabolic changes in patients with cancer lead to alterations in their amino-acid balances. Thus, amino acid profiles may be useful as biomarkers of cancers. This study was conducted to analyze amino-acid profiles in plasma in order to elucidate differences between cancer patients and controls. We analyzed the baseline serum levels of 19 amino acids in 15 patients with II and III stages of oral cancer with no metabolic alterations or other concomitant disorders and compared the results with a control group. Compared with the control group, patients with oral cancer had significant differences in lysine, ornithine, aspartic acid, serine, glycine, glutamic acid, cysteine, leucine, tyrosine, and phenylalanine. This study revealed significant differences in plasma amino acid profiles between cancer patients and controls. The development of a cancer alters plasma amino-acid profiles and the pattern of change differs between different stages of oral cancer. Plasma amino-acid profiling might therefore be useful for the early detection of cancer.

Keywords: 

amino acid profiles, plasma, screening, cancer

References: 

1. Luc A. Plasma Amino Acid Levels With a Note on Membrane Transport: Characteristics, Regulation, and Metabolic Significance. Nutrition, 2002, vol. 18, pр. 761–766.
2. Sauer L.A., Dauchy R.T. Pathways of energy metabolism in cancer. In: Watson RR, Mufti SI, editors. Nutrition and cancer prevention. Florida: CRC press; 1996. pp. 128–36.
3. Kern K.A., Norton J.A. Cancer cachexia. JPEN, 1988, vol. 12, pp. 286–298.
4. Masiar P.J., Medekova E. The role of serine and glutamine in the metabolism of malignant bone tumors and their significance in the diagnosis and prognosis of bone tumors. Neoplasm, 2002, vol. 35, pp. 197–206.
5. Watanabe A., Higashi T., Sakata T., Nagashima H. Serum amino acid levels in patients with hepatocelular carcinoma. Cancer, 1984, vol. 54, pp. 1875–1882.
6. Russell D.M., Shike M., Anderson G.H. Amino acid metabolism in small cell lung cancer. J. Parenter Enteral Nutr, 1981, vol. 6, p. 592.
7. Cascino A., Muscaritoli M., Cangiano C., et al. Plasma aminoacid imbalance in patients with lung and breast cancer. Anticancer Res, 1995, vol. 15, pp. 507–510
8. Ching N., Grossi C., Jham C. Plasma amino acid deficits and the effect of nutritional support in chemotherapy treatment. Surgery, 1984, vol. 95, pp. 730–737.
9. Tayek J.A., Sutter L., Manglik S., Lillington L.B., Grosvenor M., Chlebowski R.T. Altered metabolism and mortality in patients with colon cancer receiving chemotherapy. Am. J. Med. Sci, 1995, vol. 310, pp. 48–55.
10. Nechiporenko N.A., Nefedov L.I., Klimovich I.I. The free amino acid content of the blood serum in bladder cancer patients. Urol. Nephrol., 1990, vol. 5, pp. 17–20.
11. Elling D., Bader K., Schicke B. Free serum amino acids in patients with tumors of different sites - tumor induced imbalances. Centrally Gynecol, 1987, vol. 109, pp. 1023–1032.
12. Kuzer M., Janizewski J., Meguid M.M. Amino acid profiles in tumor-bearing and pair-fed nontumor-bearing malnourished rats. Cancer., 1988., vol. 62, pp. 30–34.
13. CoboDols M., Domínguez López M., Ramírez Plaza C., Pérez Miranda E., Gil Calle S. et al. Specific alterations in the serum amino acid profile of patients with lung cancer and head and neck cancer. Oncología, 2006, vol. 29 (7), pp. 283–290.
14. Hong-Shiee Lai, Jenq-Chang Lee, Po-Huang Lee, Shan-Tair Wang, Wei-Jao Che Plasma free amino acid profile in cancer patients. Seminars in Cancer Biology, 2005, vol. 15, pp. 267–276.
15. Johnson A.T., Kaufmann Y.C., Luo S., Todorova V., Klimberg V.S. Effect of glutamine on glutathione, IGF-I, and TGF-beta 1. J. Surg. Res., 2003, vol. 111, pp. 222–228.
16. Kaufmann Y., Luo S., Johnson A., Babb K., Klimberg V. S. Timing of oral glutamine on DMBA-induced tumorigenesis. J. Surg. Res., 2003, vol. 111, pp.158–165.
17. Lamb D.J., Puxeddu E., Malik N., Stenoien D. L., Nigam R., Saleh G. Y., et al. Molecular analysis of the androgen receptor in ten prostatecancer specimens obtained before and after androgen ablation. J. Androl., 2003, vol. 24, pp. 215–225.
18. Kubota A., Meguid M. M., Hitch D. C. Amino acid profiles correlate diagnostically with organ site in three kinds of malignant tumors. Cancer., 1992, vol. 69, pp. 2343–2348.
19. Proenza A. M., Oliver J., Palou A., Roca P. Breast and lung cancer are associated with a decrease in blood cell amino acid content. J. Nutr. Biochem, 2003, vol. 14, pp. 133–138.
20.Cascino A., Muscaritoli M., Cangiano C., et al. Plasma aminoacidimbalance in patients with lung and breast cancer. Anticancer Res., 1995, vol. 15, pp. 507–510.
21. Pisters P. W., Pearlstone D. B. Protein and amino acid metabolism incancer cachexia: investigative techniques and therapeutic interventions. Crit. Rev. Clin. Lab. Sci., 1993, vol. 30, pp. 223–272.
22. Cascino A., Muscaritoli M., Cangiano C., Conversano L., Laviano A., Ariemma S., et al. Plasma amino acid imbalance in patients with lung and breast cancer. Anticancer Res., 1995, vol. 15, pp. 507–510.
23. Georgiannos S. N., Weston P. M., Goode A. W. Correlation between albuminuria and positively charged amino acids in gastrointestinal cancer. Int. Surg., 1995, vol. 80, pp. 49–52.
23. Strauss B. S. Hypermutability and silent mutations in human carcinogenesis. Semin. Cancer Biol., 1998, vol. 8, pp. 431–438.
24. Lorite M. J., Cariuk P., Tisdale M. J. Induction of muscle protein degradationby a tumour factor. Br. J. Cancer., 1997, vol. 76, pp. 1035–1040.

Date of the publication 26.10.2013